Protective effect of grape seeds oil [Vitis Vinifera] on methomyl induced liver damage in albino rats

Liver damage was induced in adult male albino rats, weighting about 130-140 g, by oral administration of methomyl with single dose at two concentrations [1/10 or 1/20 LD[50]/day for short term [4 weeks] and long term [8 weeks]. The markers of liver damage were investigated by evaluating biochemical parameters in serum and liver tissues such as activities of aspartate aminotransferase [AST], alanine aminotransferase [ALT]], alkaline phosphatase [ALP], total bilirubin [TB], total protein [TP], total lipids [TL], total cholesterol [TC], triacylglycerols [TAG] and malondialdhyde [MDA] levels. In addition serum urea, creatinine and uric acid were assessed after 4 and 8 weeks. The effect of oral administration of grape seed oil [GSO] 4 ml/kg. Body weight/day on the above parameters for 4 and 8 weeks were investigated as a protective and antihepatotoxic effect. Oral administration of methomyl with single dose of concentration [1/10 or 1/20 LD[50]/day for 4 and 8 weeks, showed significant increase [p < 0.05] in serum liver enzymes activity [ALT, AST, ALP], TB, and MDA levels, while total protein showed a highly significant reduction as compared to negative control and control group received GSO, and the more effect was observed at the high dose of methomyl 1/lOLDso. ALT was not affected at the methomyl dose 1/20 LD[50]/day after 4 weeks. Serum lipid profile [TL, TC and TAG], creatinine and uric acid were also elevated in methomyl intoxicated rats with slight changes in TC and TL as compared to normal group at dose 1/20 LD[50]/day after 4 and 8 weeks. A significant - decrease [p < 0.05] and a high decrease [p < 0.01] in urea values were observed after short and long term in methomyl intoxicated rats. In liver tissues, elevation was found in ALP, TB, MDA, TC and TL levels after long term, while ALT, AST and total protein were significantly decreased, and intoxicated group treated with methomyl 1/10 LD[50] /day for 4 and 8 weeks was more acute effect as compared to control groups. Oral treatment with grape seed oil GSO 4 ml/kg body weight/day improved all the above parameters being almost similar to control values with treated low dose of methomyl

Dietary effect of some plants on liver functions and lipid profile in experimental animals

The effect of some plants on liver function and lipid profile was investigated. Fourty male albino rats, Sprague Dawley strain were used. The rats were divided into five groups of eight rats each with similar 156-158g.mean total weight. The rats were fed the experimental diets at 14% protein level with different types of tested plants [chicory, sonchus and moringa] for four weeks. Food and water were provided adlibitum. At the end of the experiment, rats were sacrificed under ether anesthesia and blood sample was taken from hepatic portal vein from each rat. Blood samples were subjected to the determination of serum and liver lipid profile, serum ALT, AST, ALP, MDA and erythrocyte-SOD .The results indicated that different types of tested plants had a great effect in all parametars studied. The lowest mean value for serum total lipids, triacylglycerols, total cholesterol and VLDL-cholesterol was found in group of rats fed high fat and high cholesterol diet [HFC. diet and moringa leaves] as compared with positive control group fed HFC. diet. But the lowest mean value for serum LDLcholesterol and highest mean value for serum HDL-cholesterol was found in group of rats fed [HFC diet and sonchus] as compared with positive control group. While lipid pattern in liver showed a significant decrease in group of rats fed [HFC diet and moringa leaves] as compared with positive control group and other plant groups. Also, the lowest mean value in serum ALT, AST, ALP and MDA was found in group of rats fed [HFC diet and sonchus], but the highest mean value in erythrocyte-SOD was found in group of rats fed [HFC diet and sonchus] as compared with positive control group